Description
The low molecular weight beta form of human Factor XIIa is generated in vivo by kallikrein cleavage of Factor alpha-XIIa and contains the catalytic light chain and the C-terminal peptide of the heavy chain of Factor alpha-XIIa. Recombinant Human Factor beta-XIIa is activated from insect cell culture derived Factor XII by an extended autoactivation process with Dextran Sulfate followed by repurification. Complete activation is observed on SDS-PAGE. Factor XIIa activates Factor XI to XIa thereby triggering the Contact Factor cascade. The protein purity is determined by SDS-PAGE and activity is determined via complementation in an APTT clotting assay using Factor XII immuno-deficient human plasma. Specific activity of this recombinant product is typically lower than that of plasma derived material. Custom mutants of Factor XII are also available